SAGM: preservation for RBC

Written by schmart on March 2, 2007 – 3:47 pm

Just starting to write my diploma thesis I was searching for a recipe of SAGM. After searching about 30 minutes in the Internet I decided to ask Noemi. Thanks to Noemi I can publish this recipe for SAGM solution.

For 100 ml of SAGM take

  • 0.900 g Dextrose (monohydrate)
  • 0.877 g NaCl
  • 0.0169 g Adenine
  • 0.525 g D-Mannitol
  • and dissolve it in water.

But for what are these additives good for?
In SAGM RBCs can be stored up to 42 days. It contains S = saline, A = adenine, G = glucose or dextrose, M = Mannitol. NaCl is responsible for the right osmolarity. Adenin iinfluences the ATP level in RBCs. Glucose or dextrose is essentialy for the nutrition of RBCs. Mannitol decreases the lysing rate of RBCs by supporting the integrity of the cell membrane.

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Putting sequences together

Written by schmart on February 17, 2007 – 1:39 pm

Sometimes it is a problem to open many seqeunces in one worksheet to analyse them. For examlple you have sequence data and try to open them in one worksheet in Bioedit. You can copy every sequence by copy&paste in one file or one worksheet or you can use a little helper who will do it for you. Here is one very simple helper written in Perl.
Basicaly you invoke it with an argument defining a file pattern, *.seq defines all files ending on .seq. The files should be text files. The script now reads the file contents and put the contents together in a file called sequences.fasta. Read more »


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Freeware for the Lab!

Written by schmart on February 8, 2007 – 9:00 pm

Just reading in a StudiVZ-Group about freeware for biochemistry brings me to the idea to post this:


Biobar Add-On für Mozilla Firefox:

Search in Pubmed, NCBI-Databases, EBI-Databases right from your Browser. No need to surf to the webfrontend of the service.

Labplot für Linux (KDE):
Like Origin. You can use Labplot for regression analysis, creating graphs from data and many more. Only for Linux! Read more »


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Styles, Formats and Indexes in Open Office 2.1

Written by schmart on February 2, 2007 – 2:03 pm

Ever got fed up with changeing every headline in your document by hand? Ever got fed up with update your table of content by hand? Yes? So here is a (very) short howto use OpenOffice.org Writer for doing this for you.
I decided to write this only for OpenOffice for two reasons. First OpenOffice ist OpenSource and works with more than one Operating System (for me I can’t use MS Office because I’m using Linux). Second the idea behind Styles and Formats are the same for the most Office-Software.
The first thing you have to do is to install OpenOffice on your system. Read more »


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RSS-Feeds

Written by schmart on January 30, 2007 – 12:15 pm

What is a RSS-Feed? RSS stands for Really Simple Syndication. But what is the use of RSS-Feeds? RSS-Feeds are something like a mailing list. But not the same. You can’t post to RSS-Feeds. You only can read RSS-Feeds. RSS-Feeds are XML-Documents, you can read them with a normal editor with is really silly. Or you can use a RSS-Reader. If you use a RSS-Reader you can organize your feeds. The feeds could be something like the Table Of Content from PLOS Biology, Nature, Science or Malaria Journal.
Nerly every journal has RSS-Feeds. But sometimes they are hard to find. For the most sites you can use your browsers find function and search for rss. Read more »


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